EphA4 Antibody (M280)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23328
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M280
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EphA4 Antibody (M280)
Immunogen
Clone M280 was generated from a recombinant protein corresponding to amino acids in the N-terminal region of human EphA4. This region has less than 50% homology to similar regions in other EphA and EphB family members. [UniProt# P54764]
Specificity
This antibody detects 95 and 120 kDa bands corresponding to EphA4 in Western blots of HUVEC cells and a 120 kDa band in mouse brain tissue.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for EphA4 Antibody (M280)
Western Blot: EphA4 Antibody (M280) [NBP3-23328] -
Western Blot: EphA4 Antibody (M280) [NBP3-23328] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).Applications for EphA4 Antibody (M280)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: EphA4
Long Name
Eph Receptor A4
Alternate Names
Cek8, Hek8, Sek1, Tyro1
Gene Symbol
EPHA4
Additional EphA4 Products
Product Documents for EphA4 Antibody (M280)
Product Specific Notices for EphA4 Antibody (M280)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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