ERK5/BMK1 Antibody (5C1P5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16298

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16298-100ul
NBP3-16298-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5C1P5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERK5/BMK1 Antibodies »

Product Summary for ERK5/BMK1 Antibody (5C1P5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 717-816 of human ERK5/BMK1 (Q13164). LSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK5/BMK1 Antibody (5C1P5)

Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298] - Western blot analysis of extracts of various cell lines, using ERK5/BMK1 Rabbit mAb (NBP3-16298) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Immunohistochemistry-Paraffin: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Immunohistochemistry-Paraffin: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298] - Immunohistochemistry of paraffin-embedded mouse kidney using ERK5/BMK1 Rabbit mAb (NBP3-16298) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298]

Western Blot: ERK5/BMK1 Antibody (5C1P5) [NBP3-16298] - Western blot analysis of extracts of various cell lines, using ERK5/BMK1 Rabbit mAb (NBP3-16298) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
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Applications for ERK5/BMK1 Antibody (5C1P5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK5/BMK1

The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5).

Long Name

Extracellular Signal-regulated Kinase 5

Alternate Names

BMK1, MAPK7

Gene Symbol

MAPK7

Additional ERK5/BMK1 Products

Product Documents for ERK5/BMK1 Antibody (5C1P5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK5/BMK1 Antibody (5C1P5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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