Exonuclease 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16391

Novus Biologicals, part of Bio-Techne
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NBP2-16391
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Knockdown Validated, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Exonuclease 1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Exonuclease 1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Exonuclease 1 Antibody

Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391]

Immunocytochemistry/ Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391]

Immunocytochemistry/Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391] - HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Exonuclease 1 protein stained by Exonuclease 1 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391]

Western Blot: Exonuclease 1 Antibody [NBP2-16391] - Sample (50 ug of whole cell lysate) A: mouse thymus, 7. 5% SDS PAGE gel, diluted at 1:1000.
View 1 more images

Applications for Exonuclease 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Exonuclease 1

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Alternate Names

Exo1, EXOI, Exonuclease I

Gene Symbol

EXO1

UniProt

Q9UQ84 (Human)

Additional Exonuclease 1 Products

Product Documents for Exonuclease 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Exonuclease 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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