FAM134B Antibody (PSH01-94)

Western Blot: FAM134B Antibody (PSH01-94) [NBP3-32330]

Western Blot: FAM134B Antibody (PSH01-94) [NBP3-32330] - Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (NBP3-32330) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (30 ug/Lane)
Lane 2: U-2 OS cell lysate (30 ug/Lane)
Lane 3: MCF7 cell lysate (30 ug/Lane)
Lane 4: HeLa cell lysate (30 ug/Lane)
Lane 5: HepG2 cell lysate (30 ug/Lane)
Lane 6: TT cell lysate (30 ug/Lane)
Lane 7: Jurkat cell lysate (30 ug/Lane)
Lane 8: NIH/3T3 cell lysate (30 ug/Lane)
Lane 9: C2C12 cell lysate (30 ug/Lane)
Lane 10: PC-12 cell lysate (30 ug/Lane)
Lane 11: C6 cell lysate (30 ug/Lane)
Lane 12: Mouse liver tissue lysate (30 ug/Lane)
Lane 13: Rat liver tissue lysate (30 ug/Lane)

Predicted band size: 55 kDa
Observed band size: 70 kDa

Exposure time: 6 minutes 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32330) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: FAM134B Antibody (PSH01-94) [NBP3-32330]

Immunocytochemistry/ Immunofluorescence: FAM134B Antibody (PSH01-94) [NBP3-32330] - Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (NBP3-32330) at 1/100 dilution.

Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (NBP3-32330) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.