Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08997
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # AT130-5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
CD32-rat CD4 domain 3/4 fusion protein.
Specificity
This antibody is specific for murine Fc gamma RII/CD32, which is a Fc gamma receptor that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. Fc gamma RII/CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free [NBP3-08997]](https://resources.bio-techne.com/images/products/Fc-gamma-RII-CD32-Antibody-AT130-5-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-08997-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free [NBP3-08997]")
Immunocytochemistry/ Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free [NBP3-08997]
Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.Applications for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
The antibody binds to CD32, a surface receptor protein with a low affinity for IgG antibodies.
The antibody binds to CD32, a surface receptor protein with a low affinity for IgG antibodies.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fc gamma RII/CD32
Long Name
Fc gamma Receptor II
Alternate Names
FcgRII
Gene Symbol
FCGR2B
Additional Fc gamma RII/CD32 Products
Product Documents for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free
Product Specific Notices for Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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