FCHSD1 Antibody (4G4)

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01]

Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 and FCHSD2 immunolocalization in mouse cochlear hair cells. Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01]

Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells. Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.

Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] -

Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Expression analysis of mouse Fchsd1 & Fchsd2 in different tissues.(A) Total RNA from postnatal day 5 mouse tissues was extracted & used as template for reverse transcription. PCR was performed using this cDNA as template. Upper panel, Fchsd1 mRNA is expressed more abundantly in nervous tissues. Middle panel, Fchsd2 mRNA is expressed ubiquitously in all tissues examined. Lower panel, beta-actin specific primers were used as the RT-PCR template control. (B) Total proteins of postnatal day 5 mouse cochlea & vestibula were extracted & separated by PAGE & detected with antibodies against FCHSD1(Novus), FCHSD2, or SNX9. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] -

Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Expression analysis of mouse Fchsd1 & Fchsd2 in different tissues.(A) Total RNA from postnatal day 5 mouse tissues was extracted & used as template for reverse transcription. PCR was performed using this cDNA as template. Upper panel, Fchsd1 mRNA is expressed more abundantly in nervous tissues. Middle panel, Fchsd2 mRNA is expressed ubiquitously in all tissues examined. Lower panel, beta-actin specific primers were used as the RT-PCR template control. (B) Total proteins of postnatal day 5 mouse cochlea & vestibula were extracted & separated by PAGE & detected with antibodies against FCHSD1(Novus), FCHSD2, or SNX9. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] -

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] -

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 & FCHSD2 immunolocalization in mouse cochlear hair cells.Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. (A) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. (B) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) FCHSD2 immunoreactivity in the hair bundles of 6-week old mouse cochlear hair cells. (D) FCHSD2 immunoreactivity in the hair bundles of 7-month old mouse inner hair cells. (E) FCHSD2 immunoreactivity in the hair bundles of 2-week old mouse outer hair cells. The Novus FCHSD1 antibody was used in (A) & (B). Scale bars: 10 µm in(A-C), 5 µm in (D) & (E). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] -

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] -

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 & FCHSD2 immunolocalization in mouse cochlear hair cells.Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. (A) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. (B) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) FCHSD2 immunoreactivity in the hair bundles of 6-week old mouse cochlear hair cells. (D) FCHSD2 immunoreactivity in the hair bundles of 7-month old mouse inner hair cells. (E) FCHSD2 immunoreactivity in the hair bundles of 2-week old mouse outer hair cells. The Novus FCHSD1 antibody was used in (A) & (B). Scale bars: 10 µm in(A-C), 5 µm in (D) & (E). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] -

Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.