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FXR2 Antibody (1G2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-259

Novus Biologicals, part of Bio-Techne
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NB300-259
NB300-259SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G2

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant C-terminal fragment of human FXR2 (residues 414-658). [UniProt# P51116]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FXR2 Antibody (1G2) - BSA Free

Western Blot: FXR2 Antibody (1G2)BSA Free [NB300-259]

Western Blot: FXR2 Antibody (1G2)BSA Free [NB300-259]

Western Blot: FXR2 Antibody (1G2) [NB300-259] - Whole cell HeLa lysate at a concentration of 1:2500 (lane 1) and 1:500 (lane 2).
Immunocytochemistry/ Immunofluorescence: FXR2 Antibody (1G2) - BSA Free [NB300-259]

Immunocytochemistry/ Immunofluorescence: FXR2 Antibody (1G2) - BSA Free [NB300-259]

Immunocytochemistry/Immunofluorescence: FXR2 Antibody (1G2) [NB300-259] - FXR2 (1G2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for FXR2 Antibody (1G2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

20 ug/ml

Immunohistochemistry-Paraffin

20 ug/ml

Western Blot

1:500-1:2500
Application Notes
This FXR2 Antibody (1G2) is useful for Immunohistochemistry on paraffin-embedded secitons and Western blot, where a band is seen at ~80 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FXR2

FXR2 (Fragile X relative protein 2) belongs to FMR1 family/fragile X mental retardation proteins which can bind to RNA and associate with polyribosomes. All three members of FMR1 proteins i.e. FMRP, FXR1, and FXR2 contains RNA-binding domains and RGG boxes, and are found predominantly in cytoplasm where they can associate with ribosomes for implicating translation or mRNA stability. FXR2 has been shown to interact with FMR1, FXR1, CYFIP2 and TDRD3. It is ubiquitously expressed during embryonic development and its tissue distribution overlaps with FMRP in adult stages. However, interestingly, FXR2 knockout mice display learning impairments and changes in synaptic plasticity that are somewhat distinct from those of FMRP-deficient mice, indicating that FXR2 may also regulate adult hippocampal neurogenesis via mechanisms distinct from FMRP. FXR2 deficiency leads to altered stem cell proliferation and differentiation specifically in dentate gyrus and not in subventricular zone which has been suggested as a key component of mechanism regulating differential neurogenic processes in these two adult germinal zones.

Alternate Names

FMR1L2, fragile X mental retardation syndrome-related protein 2, fragile X mental retardation, autosomal homolog 2, fragile X-mental retardation 1-like 2, FXR2P

Gene Symbol

FXR2

Additional FXR2 Products

Product Documents for FXR2 Antibody (1G2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FXR2 Antibody (1G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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