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G9a/EHMT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42846

Novus Biologicals, part of Bio-Techne
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NBP2-42846

Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (98%), Herring (98%), Porcine (98%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for G9a/EHMT2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse G9a/EHMT2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G9a/EHMT2 Antibody

Western Blot: G9a/EHMT2 Antibody [NBP2-42846]

Western Blot: G9a/EHMT2 Antibody [NBP2-42846]

Western Blot: G9a/EHMT2 Antibody [NBP2-42846] - Analysis of 30 ug whole cell lysate. A:NIH-3T3 5% SDS PAGE diluted at 1:1000.

Applications for G9a/EHMT2 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Gene Symbol

EHMT2

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for G9a/EHMT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.