G9a/EHMT2 Antibody

Western Blot: G9a/EHMT2 Antibody [NBP3-18664]

Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2 , diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

ELISA: G9a/EHMT2 Antibody [NBP3-18664]

ELISA: G9a/EHMT2 Antibody [NBP3-18664] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a/EHMT2 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.