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GAT-1/SLC6A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26059

Novus Biologicals, part of Bio-Techne
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NBP2-26059

Key Product Details

Species Reactivity

Human, Rat, Canine

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-EQPQAGSSTSKE corresponding to C-Terminus according to NP_003033.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Applications for GAT-1/SLC6A1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave bands at approx. 50 kDa band and 37 kDa band in human brain (cerebellum) lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 67.1 kDa band according to NP_003033.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAT-1/SLC6A1

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Long Name

GABA Transporter 1

Alternate Names

GABATHG, GABATR, GAT1, SLC6A1

Entrez Gene IDs

6529 (Human); 79212 (Rat)

Gene Symbol

SLC6A1

Additional GAT-1/SLC6A1 Products

Product Documents for GAT-1/SLC6A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GAT-1/SLC6A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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