GLYCTK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12587

Novus Biologicals, part of Bio-Techne
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NBP3-12587-100ul
NBP3-12587-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GLYCTK Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human GLYCTK fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for GLYCTK Antibody

Western Blot: GLYCTK Antibody [NBP3-12587]

Western Blot: GLYCTK Antibody [NBP3-12587]

Western Blot: GLYCTK Antibody [NBP3-12587] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: GLYCTK Antibody [NBP3-12587]

Immunocytochemistry/ Immunofluorescence: GLYCTK Antibody [NBP3-12587]

Immunocytochemistry/Immunofluorescence: GLYCTK Antibody [NBP3-12587] - Staining of GLYCTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GLYCTK polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP3-12587]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP3-12587]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP3-12587] - Staining of human GLYCTK in human kidney with rabbit polyclonal antibody at 1:2000 dilution.
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Applications for GLYCTK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLYCTK

GLYCTK, also known as Glycerate kinase, has 7 isoforms, a 523 amino acid isoform 1 that is 55 kDa, a 234 amino acid isoform 2 that is 25 k Da, a 457 amino acid isoform 3 that is 48 kDa, a 367 amino acid isoform 4 that is 39 kDa, a 240 amino acid isoform 5 that is 25 kDa, a 205 amino acid isoform 6 that is 22 kDa, and a 187 amino acid isoform 7 that is 20 kDa; acts as a catalyzer of the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. This protein is being studied for its involvement in microcephaly and seizures. GLYCTK has been linked to glycine, serine and threonine metabolism, glycerolipid metabolism, and glyoxylate and dicarboxylate metabolism pathways where interacts with TRIP13, ALDH1B1, ALDH2, ALDH7A1, and GRHPR proteins.

Alternate Names

EC 2.7.1.31, glycerate kinase, GLYCTK1, HBeAg-binding protein 2, HBeAg-binding protein 4, HBeAgBP4A, HBEBP2, HBEBP4HBeAg binding protein 4

Gene Symbol

GLYCTK

Additional GLYCTK Products

Product Documents for GLYCTK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GLYCTK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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