HDAC4+5+9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04967

Novus Biologicals, part of Bio-Techne
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NBP1-04967-0.025ml
NBP1-04967
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Drosophila

Cited:

Insect - Drosophila

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HDAC4+5+9 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around amino acids 244~248/257~261/218~222 (T-A-S-E-P)

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 25329053)

Specificity

Detects endogenous levels of total HDAC4+5+9 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC4+5+9 Antibody

Immunocytochemistry/ Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967]

Immunocytochemistry/ Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967]

Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.

Immunohistochemistry-Paraffin: HDAC4+5+9 Antibody [NBP1-04967] -

Immunohistochemistry-Paraffin: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using NBP1-04967 (left) or the same antibody preincubated with blocking peptide (right).
HDAC4+5+9 Antibody

Western Blot: HDAC4+5+9 Antibody [NBP1-04967] -

Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Western blot analysis of extracts from 293 and Hela cells using NBP1-04967

Applications for HDAC4+5+9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDAC4+5+9

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.

Alternate Names

HA6116, HD4EC 3.5.1.98, HDAC-A, HDACABDMR, histone deacetylase 4, histone deacetylase A, KIAA0288AHO3

Entrez Gene IDs

9759 (Human); 208727 (Mouse)

Gene Symbol

HDAC4

UniProt

P56524 (Human)

Additional HDAC4+5+9 Products

Product Documents for HDAC4+5+9 Antibody

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Certificate of Analysis

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Product Specific Notices for HDAC4+5+9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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