HIST3H3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24898

Novus Biologicals, part of Bio-Techne
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NBP3-24898
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIST3H3 Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein HIST3H3 using the following amino acid sequence: MARTKQTARKSTGGKAPRKQLATKVARKS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIST3H3 Antibody

HIST3H3 Antibody Immunocytochemistry/Immunofluorescence: HIST3H3 Antibody [NBP3-24898]

Immunocytochemistry/Immunofluorescence: HIST3H3 Antibody [NBP3-24898]

Staining of human cell line MCF-7 shows localization to nucleoplasm.

Applications for HIST3H3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIST3H3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The HIST3H3 gene is intronless and encodes a 136 amino acid long, 15 kDA histone H3.1t protein that lacks polyA tails and obtain a palindromic termination element. The HIST3H3 gene is located separately from the other H3 genes on the cluster on chromosome 6p22-p21.3. HIST3H3 participates in PKA signaling, development notch signaling pathway, histone modification, and interferon pathway through interactions with genes HIST1H4B, HIST4H4, HIST1H4A, HIST1H4C, and HIST1H4D. HIST3H3 has been researched regarding its role in immunodeficiency, leukemia, prostatitis, huntington's disease, lupus, multiple sclerosis, laryngeal cancer, and lymphomas.

Long Name

Histone Cluster 3 H3

Alternate Names

H3.4, H3/G, H3/T, H3FT, H3t, Histone 3, H3

Gene Symbol

H3-4

Additional HIST3H3 Products

Product Documents for HIST3H3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIST3H3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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