Histone H2B type 1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98972
![Western Blot: Histone H2B type 1B Antibody [NBP2-98972]](https://resources.bio-techne.com/images/products/Histone-H2B-type-1B-Antibody-Western-Blot-NBP2-98972-img0003.jpg "Western Blot: Histone H2B type 1B Antibody [NBP2-98972]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Histone H2B type 1B.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Histone H2B type 1B Antibody
Western Blot: Histone H2B type 1B Antibody [NBP2-98972]
Western Blot: Histone H2B type 1B Antibody [NBP2-98972] - Anti-Histone H2B type 1B rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 15 kDa![Immunoprecipitation: Histone H2B type 1B Antibody [NBP2-98972]](https://resources.bio-techne.com/images/products/Histone-H2B-type-1B-Antibody-Immunoprecipitation-NBP2-98972-img0004.jpg "Immunoprecipitation: Histone H2B type 1B Antibody [NBP2-98972]")
Immunoprecipitation: Histone H2B type 1B Antibody [NBP2-98972]
Immunoprecipitation: Histone H2B type 1B Antibody [NBP2-98972] - Histone H2B type 1B was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 1 ul anti-Histone H2B type 1B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Histone H2B type 1B rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 25 kDaApplications for Histone H2B type 1B Antibody
Application
Recommended Usage
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2B type 1B
Alternate Names
H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804
Gene Symbol
H2BC3
Additional Histone H2B type 1B Products
Product Documents for Histone H2B type 1B Antibody
Product Specific Notices for Histone H2B type 1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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