Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HJURP Antibodies »

Product Summary for HJURP Antibody

Immunogen

The immunogen for this product maps to a region between residue 650 and 700 of human Holliday Junction Recognition Protein using the numbering given in entry NP_060880.3 (GeneID 55355).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HJURP Antibody

Western Blot: HJURP Antibody [NBP1-47287]

Western Blot: HJURP Antibody [NBP1-47287]

Western Blot: HJURP Antibody [NBP1-47287] - Detection of human HJURP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HJURP antibody NBP1-47287 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HJURP was also immunoprecipitated by rabbit anti-HJURP antibody NBP1-472878, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
HJURP Antibody [NBP1-47287]

HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB

HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB
HJURP Antibody [NBP1-47287]

HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

Applications for HJURP Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HJURP

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions

Alternate Names

FAKTS14-3-3-associated AKT substrate, fetal liver expressing gene 1, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1, Holliday junction recognition protein, Up-regulated in lung cancer 9, URLC9DKFZp762E1312

Entrez Gene IDs

55355 (Human)

Gene Symbol

HJURP

Additional HJURP Products

Product Documents for HJURP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HJURP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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