HLA DRB1 Antibody (6Z1F10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15950
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6Z1F10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HLA DRB1 Antibody (6Z1F10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HLA DRB1 (P01911). ARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HLA DRB1 Antibody (6Z1F10)
Western Blot: HLA DRB1 Antibody (6Z1F10) [NBP3-15950]
Western Blot: HLA DRB1 Antibody (6Z1F10) [NBP3-15950] - Western blot analysis of extracts of various cell lines, using HLA DRB1 antibody (NBP3-15950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry-Paraffin: HLA DRB1 Antibody (6Z1F10) [NBP3-15950]
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (6Z1F10) [NBP3-15950] - Immunohistochemistry of paraffin-embedded human colon using HLA DRB1 Rabbit mAb (NBP3-15950) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for HLA DRB1 Antibody (6Z1F10)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HLA DRB1
Alternate Names
Clone P2-beta-3, DR1, DR-1, DR12, DR-12, DR13, DR-13, DR14, DR-14, DR16, DR-16, DR4, DR-4, DR5, DR-5, DR7, DR-7, DR8, DR-8, DR9, DR-9, DRB1, DRw10, DRw11, DRw8, DW2.2/DR2.2, FLJ75017, FLJ76359, HLA class II antigen beta chain, HLA class II histocompatibility antigen, DR-1 beta chain, HLA-DR1B, HLA-DRB, HLA-DRB1*, HLA-DRB2, HLA-DR-beta 1, human leucocyte antigen DRB1, leucocyte antigen DR beta 1 chain, leucocyte antigen DRB1, lymphocyte antigen DRB1, major histocompatibility complex, class II, DR beta 1, MHC class II antigen DRB1*1, MHC class II antigen DRB1*10, MHC class II antigen DRB1*11, MHC class II antigen DRB1*12, MHC class II antigen DRB1*13, MHC class II antigen DRB1*14, MHC class II antigen DRB1*15, MHC class II antigen DRB1*16, MHC class II antigen DRB1*3, MHC class II antigen DRB1*4, MHC class II antigen DRB1*7, MHC class II antigen DRB1*8, MHC class II antigen DRB1*9, MHC class II antigen HLA-DR13, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR-beta cell surface glycoprotein, MHC class II HLA-DRw10-beta, SS1
Gene Symbol
HLA-DRB1
Additional HLA DRB1 Products
Product Documents for HLA DRB1 Antibody (6Z1F10)
Product Specific Notices for HLA DRB1 Antibody (6Z1F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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