HOIP/RNF31 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55059
Key Product Details
Species Reactivity
Validated:
Human
Applications
Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Summary for HOIP/RNF31 Antibody
Immunogen
Synthetic peptides corresponding to RNF31(ring finger protein 31) The peptide sequence was selected from the middle region of RNF31. Peptide sequence SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for HOIP/RNF31 Antibody
Western Blot: HOIP/RNF31 Antibody [NBP1-55059]
Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysateWestern Blot: HOIP/RNF31 Antibody [NBP1-55059]
Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Lanes: Lane1 : 50 ug hormoxia A549 lysate Lane2: 50 ug hypoxia A549 lysate Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA) Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA) Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA) Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA) Primary, Antibody Dilution: 1 : 800 Secondary Antibody: Goat anti rabbit HRP Secondary, Antibody Dilution: 1 : 10000 Gene name: RNF31.Western Blot: HOIP/RNF31 Antibody [NBP1-55059]
Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Sample Tissue: Human MCF7 Antibody Dilution: 1.0 ug/mlApplications for HOIP/RNF31 Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HOIP/RNF31
Additional HOIP/RNF31 Products
Product Documents for HOIP/RNF31 Antibody
Product Specific Notices for HOIP/RNF31 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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