I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?

I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.

Does HPa2 cross react with other species? Specifically mouse or monkey?

NBP1-18950 hasn't been tested in mouse or monkey but is predicted to cross react with both. It is not guaranteed for use in these species, but if you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program. Under this program by completing an online review with image, detailing your positive or negative results, you receive a discount voucher for 100% of the purchase price of the reviewed product.

HPa2 Antibody (DHIC2-2B4)

Name: HPa2 Antibody (DHIC2-2B4)
Brand: Novus Biologicals
SKU: NBP1-18950

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18950

Submit a review

Citations (6)

Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP1-18950AF350

View Product

Alexa Fluor 405

NBP1-18950AF405

View Product

Alexa Fluor 488

NBP1-18950AF488

View Product

Alexa Fluor 532

NBP1-18950AF532

View Product

Alexa Fluor 594

NBP1-18950AF594

View Product

Alexa Fluor 647

NBP1-18950AF647

View Product

Alexa Fluor 700

NBP1-18950AF700

View Product

Alexa Fluor 750

NBP1-18950AF750

View Product

Allophycocyanin

NBP1-18950APC

View Product

Allophycocyanin/Cy7

NBP1-18950APCCY7

View Product

Biotin

NBP1-18950B

View Product

CoraFluor 1

NBP1-18950CL1

View Product

DyLight 350

NBP1-18950UV

View Product

DyLight 405

NBP1-18950V

View Product

DyLight 488

NBP1-18950G

View Product

DyLight 550

NBP1-18950R

View Product

DyLight 594

NBP1-18950DL594

View Product

DyLight 650

NBP1-18950C

View Product

DyLight 680

NBP1-18950FR

View Product

DyLight 755

NBP1-18950IR

View Product

FITC

NBP1-18950F

View Product

HRP

NBP1-18950H

View Product

Janelia Fluor 525

NBP1-18950JF525

View Product

Janelia Fluor 549

NBP1-18950JF549

View Product

Janelia Fluor 585

NBP1-18950JF585

View Product

Janelia Fluor 635

NBP1-18950JF635

View Product

Janelia Fluor 646

NBP1-18950JF646

View Product

Janelia Fluor 669

NBP1-18950JF669

View Product

mFluor Violet 450 SE

NBP1-18950MFV450

View Product

mFluor Violet 500 SE

NBP1-18950MFV500

View Product

mFluor Violet 610 SE

NBP1-18950MFV610

View Product

NBP1-18950PE

View Product

PE/Atto594

NBP1-18950PEATT594

View Product

PE/Cy5.5

NBP1-18950PECY55

View Product

PE/Cy7

NBP1-18950PECY7

View Product

PerCP

NBP1-18950PCP

View Product

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # DHIC2-2B4

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all HPa2 Antibodies »

Product Specifications

Immunogen

Human pancreatic enriched alpha cells

Localization

Cell surface

Specificity

Specific for alpha endocrine cell types.

Marker

Alpha Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for HPa2 Antibody (DHIC2-2B4)

Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]

Immunocytochemistry/Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.

Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]

Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Analysis of enzyme dispersed human pancreas cells.

Applications for HPa2 Antibody (DHIC2-2B4)

Application

Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Western Blot

1:100-1:2000

Application Notes

This HPa2 (DHIC2-2B4) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence, Flow cytometry and Western blot.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HPa2

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Alternate Names

human pancreatic alpha cell

Additional HPa2 Products

Product Documents for HPa2 Antibody (DHIC2-2B4)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for HPa2 Antibody (DHIC2-2B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

HPa2 Antibody (DHIC2-2B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18950

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all HPa2 Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Marker

Clonality

Host

Isotype

Scientific Data Images for HPa2 Antibody (DHIC2-2B4)

Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]

Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950]

Applications for HPa2 Antibody (DHIC2-2B4)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: HPa2

Alternate Names

Additional HPa2 Products

Product Documents for HPa2 Antibody (DHIC2-2B4)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for HPa2 Antibody (DHIC2-2B4)