Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1039F

R&D Systems, part of Bio-Techne
Discontinued Product
FAB1039F has been discontinued. View all 2B4/CD244/SLAMF4 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

View all 2B4/CD244/SLAMF4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human 2B4/CD244/SLAMF4
Met1-Arg221
Accession # NP_057466

Specificity

Detects human 2B4/CD244/SLAMF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse (rm) 2B4, recombinant human (rh) SLAM, rhCD48, and rhCD84 is observed. In direct ELISAs and Western blots, less than 5% cross‑reactivity with rm2B4 and rhSLAM is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antibody

Detection of 2B4/CD244/SLAMF4 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of 2B4/CD244/SLAMF4 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1039F) or (B) Normal Goat IgG Fluorescein Control (Catalog # IC108F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1-4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%-24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma delta T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8-10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7-13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15).

References

  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
  4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  5. Boles, K.S. et al. (1999) Tissue Antigens 54:27.
  6. Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466.
  7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
  8. Lee, K.M. et al. (2006) Blood 107:3181.
  9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
  10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
  11. Bryceson, Y.T. et al. (2006) Blood 107:159.
  12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
  13. McNerney, M.E. et al. (2005) Blood 106:1337.
  14. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870.
  15. Messmer, B. et al. (2006) J. Immunol. 176:4646.

Alternate Names

CD244, NAIL, NKR2B4, Nmrk, SLAMF4

Entrez Gene IDs

51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey)

Gene Symbol

CD244

UniProt

NP_057466

Additional 2B4/CD244/SLAMF4 Products

Product Documents for Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antibody

Product Datasheets

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Product Specific Notices for Human 2B4/CD244/SLAMF4 Fluorescein-conjugated Antibody

For research use only

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