Human BMI-1 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC33341A
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 384515
Product Specifications
Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Asp96-Gly326
Accession # P35226
Specificity
Detects human BMI-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human BMI-1 APC-conjugated Antibody
Detection of BMI-1 in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human BMI-1 APC-conjugated Monoclonal Antibody (Catalog # IC33341A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human BMI-1 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BMI-1
BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa proto-oncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain within amino acids (aa) 17-56, a nuclear location sequence (NLS) at aa 81-95 and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.
Long Name
B Lymphoma Mo-MLV Insertion Region 1
Alternate Names
BMI1, PCGF4, RNF51
Gene Symbol
BMI1
UniProt
Additional BMI-1 Products
Product Documents for Human BMI-1 APC-conjugated Antibody
Product Specific Notices for Human BMI-1 APC-conjugated Antibody
For research use only
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