| Specificity: |
Detects a synthetic peptide corresponding to residues near amino acid 50 of human GLUT1 protein by Direct ELISA.
Applications for Human Glut1 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
Jurkat human acute T cell leukemia cell line, K562 chronic myelogenous leukemia cell line, and MDA-MB-231 human breast cancer cell line |
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Immunohistochemistry |
3-25 µg/mL |
Immersion fixed paraffin-embedded sections of human lung cancer and sections of human pancreatic cancer |
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Immunocytochemistry |
3-25 µg/mL |
Immersion fixed HepG2 human hepatocellular carcinoma cell line and human PBMC |
Scientific Data Examples for Human Glut1 Antibody
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Western Blot
Detection of Human Glut1 by Western Blot.
Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glut1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunocytochemistry
Detection of Glut1 in HepG2 Human Cell Line.
Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line and absent in human PBMC cells using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface of HepG2 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunohistochemistry
Detection of Glut1 in Human Lung Cancer.
Glut1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Immunohistochemistry
Detection of Glut1 in Human Pancreatic Cancer.
Glut1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB11601) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human Glut1 Antibody
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