Species Reactivity:

Human

Immunogen:

NS0 mouse myeloma cell line transfected with human GPRC5D
Met1-Val359
Accession # Q9NZD1

Applications:

CyTOF-ready, Flow Cytometry

Label:

Unconjugated

Source:

Monoclonal Mouse IgG_2A Clone # 571932

Specificity:

Detects human GPRC5D in direct ELISAs.

View Full Technical Details for Human GPRC5D Antibody

Technical Data
Citations / Reviews

Applications for Human GPRC5D Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human GPRC5D Antibody

	Flow Cytometry Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) Human GPRC5D or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB63001) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Item	Value
Long Name	G Protein-coupled Receptor, Family D, Group 5, Member D
Entrez Gene IDs	55507 (Human); 93746 (Mouse); 500349 (Rat)
Alternate Names	G protein-coupled receptor, family C, group 5, member D, G-protein coupled receptor family C group 5 member D, GPRC5D, MGC129713, MGC129714, orphan G-protein coupled receptor

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Human GPRC5D Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB63001

Key Product Details

Applications for Human GPRC5D Antibody

Scientific Data Examples for Human GPRC5D Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: GPRC5D

Customer Reviews for Human GPRC5D Antibody

Product Documents for Human GPRC5D Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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