Human HNF-4 alpha/NR2A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # PP-H1415-00
Isoforms 1, 2, 4, 5, 7, 8
Discontinued Product
PP-H1415-00 has been discontinued.
An alternative/replacement product is available:
PP-H1415-0C. View all HNF-4 alpha/NR2A1 products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Direct ELISA, Gel Supershift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # H1415
Product Specifications
Immunogen
Recombinant human HNF-4 alpha/NR2A1
aa 393-461
aa 393-461
Specificity
This antibody specifically recognizes human HNF4 alpha (isoforms 1, 2, 4, 5, 7
and 8) and cross-reacts with mouse and rat HNF4 alpha (isoforms 1, 2, 4, 5, 7
and 8). Not yet tested in other species.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human HNF-4 alpha/NR2A1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions should be determined by
each laboratory.
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriate
secondary reagents to detect human HNF4 alpha.
Gel Supershift Assay
This antibody can be used at 2 ng/assay to detect human
HNF4 alpha.
Immunohistochemistry
This antibody can be used at 10 - 20 μg/mL with
the appropriate secondary reagents to detect human HNF4 alpha.
Immunoprecipitation
Optimal dilutions should be determined by each
laboratory.
Western Blot
This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human HNF4 alpha. Under non-reducing conditions, optimal dilutions should be determined by each laboratory.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a
manual defrost freezer or at -70 °C. Upon thawing, the antibody can be
stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid
repeated freeze-thaw cycles.
Background: HNF-4 alpha/NR2A1
Long Name
Hepatocyte Nuclear Factor-4, alpha
Alternate Names
HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14
Entrez Gene IDs
3172 (Human)
Gene Symbol
HNF4A
Additional HNF-4 alpha/NR2A1 Products
Product Documents for Human HNF-4 alpha/NR2A1 Antibody
Product Specific Notices for Human HNF-4 alpha/NR2A1 Antibody
For research use only
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