Human Kallikrein 3/PSA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1344

R&D Systems, part of Bio-Techne
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MAB1344-100
MAB1344-500
MAB1344-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

ELISA Microarray Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 181823

View all Kallikrein 3/PSA Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Ala18-Pro261
Accession # P07288

Specificity

Detects human Kallikrein 3/PSA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Kallikrein 5 or 11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Kallikrein 3/PSA Antibody

Detection of Human Kallikrein 3/PSA antibody by Western Blot.

Detection of Human Kallikrein 3/PSA by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB1344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 3/PSA antibody in LNCaP and MCF-7 Human Cell Lines by Immunocytochemistry (ICC).

Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines.

Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB1344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Kallikrein 3/PSA antibody in Prostate Cancer by Immunohistochemistry (IHC-P).

Kallikrein 3/PSA in Prostate Cancer.

Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of prostate cancer tissue using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB1344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the apical plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Kallikrein 3/PSA Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded prostate cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human prostate tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1344 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5-2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer. The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). Recombinant human PSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha1-antichymotrypsin (ACT) and alpha2-macroglobulin are known to form complexes with PSA.

References

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
  3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
  4. Lilja H. (2003) Urology 62:270.
  5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.

Alternate Names

KLK3, PSA

Entrez Gene IDs

354 (Human)

Gene Symbol

KLK3

UniProt

P07288

Additional Kallikrein 3/PSA Products

Product Documents for Human Kallikrein 3/PSA Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 3/PSA Antibody

For research use only

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