Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2187
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 308320
Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219
Ala2-Gln290
Accession # P35219
Specificity
Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody
Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody (Catalog # MAB2187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain (cerebellum) tissue and mouse brain (cerebellum) tissue
Sample: Human brain (cerebellum) tissue and mouse brain (cerebellum) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase VIII/CA8
Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
- Hirota, J. et al. (2003) Biochem. J. 372: 435.
- Miyaji, E. et al. (2003) J. Pathol. 201: 37.
- Lu, S.H. et al. (2004) Lung Cancer 44: 273.
Alternate Names
CA8, CALS, CARP
Entrez Gene IDs
767 (Human)
Gene Symbol
CA8
UniProt
Additional Carbonic Anhydrase VIII/CA8 Products
Product Documents for Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody
Product Specific Notices for Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody
For research use only
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