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Human/Mouse/Hamster GDF-9 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF739

R&D Systems, part of Bio-Techne
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AF739
AF739-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-9

Specificity

Detects mouse GDF‑9 in direct ELISAs and human, mouse and hamster GDF-9 in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) GDF-1, rmGDF-5, rmGDF-6, rmGDF-8, and rmGDF-15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Hamster GDF-9 Antibody

Detection of Mouse and Hamster GDF-9 antibody by Western Blot.

Detection of Human/Hamster GDF-9 by Western Blot.

Western blot shows lysates of human ovary tissue and CHO Chinese hamster ovary cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Hamster GDF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for GDF-9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GDF-9 antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr).

GDF‑9 in Mouse Ovary.

GDF-9 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Human/Mouse/Hamster GDF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF739) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing oocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse GDF-9 by Western Blot.

Western Blot shows lysates of mouse ovary. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse/Hamster GDF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF-9 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human/Mouse/Hamster GDF-9 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11-13) and perfusion fixed frozen sections of mouse ovary

Western Blot

1 µg/mL
Sample: Human ovary tissue, mouse ovary tissue, and CHO Chinese hamster ovary cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-9

Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor-beta (TGF-beta) superfamily, and is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis (1‑2). Mouse GDF-9 is synthesized as a 441 amino acid (aa) prepropeptide that contains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa mature chain. Residues 340‑441 constitute a TGF-beta like domain. In addition, there is one potential site of N-linked glycosylation in the mature chain. Unlike other members of the TGF-beta superfamily, GDF‑9 lacks the conserved cysteine residue that is believed to form the sole disulfide linkage between subunits in other family members (3). Mature mouse GDF-9 shares 90% aa sequence identity with mature human GDF 9. The protein is expressed throughout the development of the maturing follicle (2). GDF-9 functions as a paracrine factor in the regulation of granulosa cell proliferation and differentiation, and is essential for fertility (2, 4). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (5). Mouse GDF-9 induces Smad2 phosphorylation and inhibin production in rat diethylstilbestrol treated granulosa cells (6) and in human granulosa-luteal cells (7). The downstream signaling actions of GDF 9 are mediated by the type I receptor, activin receptor-like kinase 5 (ALK5), initiating the subsequent activation of Smad2 and Smad3 (2, 8). GDF 9 uses the BMP type II receptor (BMPRII) as its other signaling receptor (2, 9).

References

  1. McGrath, S.A. et al. (1995) Mol. Endocrinol. 9:131.
  2. Mottershead, D.G. et al. (2008) Mol. Cell. Endocrinol. 283:58.
  3. McPherron, A.C. and S.-J. Lee (1992) J. Biol. Chem. 268:3444.
  4. Gilchrist, R.B. et al. (2006) J. Cell. Sci. 119:3811.
  5. Dong, J. et al. (1996) Nature 383:531.
  6. Roh, J.S. et al. (2003) Endocrinology 144:172.
  7. Kaivo-Oja, N. et al. (2003) J. Clin. Endocrinol. Metab. 88:755.
  8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
  9. Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.

Long Name

Growth Differentiation Factor 9

Alternate Names

GDF9

Entrez Gene IDs

2661 (Human); 14566 (Mouse)

Gene Symbol

GDF9

Additional GDF-9 Products

Product Documents for Human/Mouse/Hamster GDF-9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Hamster GDF-9 Antibody

For research use only

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