Human/Mouse IkB-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5225
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse I kappaB-beta
Met1-Ala359
Accession # Q60778
Met1-Ala359
Accession # Q60778
Specificity
Detects endogenous human and mouse I kappaB-beta in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse IkB-beta Antibody
Detection of Mouse IkB-beta by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse IkB-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5225) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IkB-beta at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse IkB-beta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line, BaF3 mouse pro-B cell line, M1 mouse myeloid leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-beta
I kappaB-beta (I kappa B-beta; also NF-kappa-BIB) is a 46 kDa member of the I kappaB-beta inhibitor family of proteins. It is highly expressed in testicular spermatids, and serves to block NF-kappa B activity. This is accomplished by I kappaB-beta binding to either an NF-kappa B dimer, or a kappaB-Ras:NF-kappa B dimer complex. Mouse I kappaB-beta is 359 amino acids (aa) in length it contains a signal response region (aa 1‑50), six ankyrin repeats (aa 54‑308) and a PEST domain whose phosphorylation at Ser312 and Ser314 blocks NF‑ kappaB activation. Phosphorylation at Ser19 and Ser23 promotes I kappaB‑ beta ubiquitination and degradation. Full‑length mouse I kappaB‑ beta shares 94% and 84% aa identity with rat and human I kappaB-beta, respectively.
Long Name
I-kappa-B-beta
Alternate Names
IkBbeta, NFKBIB, TR-interacting protein 9
Gene Symbol
NFKBIB
UniProt
Additional IkB-beta Products
Product Documents for Human/Mouse IkB-beta Antibody
Product Specific Notices for Human/Mouse IkB-beta Antibody
For research use only
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