Human/Mouse/Rat Integrin alphaV/CD51 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1219
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Flow Cytometry, Flow Cytometry Control, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alphaV/CD51
Phe31-Val992
Accession # P06756
Phe31-Val992
Accession # P06756
Specificity
Detects human Integrin alphaV in direct ELISAs and Western blots. Detects human, mouse and rat Integrin alphaV in Simple Western application.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Integrin alphaV/CD51 Antibody
Detection of Human Integrin alphaV/CD51 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Integrin alphaV/CD51 by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin alphaV/CD51 at approximately 150 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin alphaV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Integrin alphaV/CD51 in Rat Mesenchymal Stem Cells.
Integrin aV/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Integrin alphaV/CD51 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells
Sample: Human peripheral blood mononuclear cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed DU145 human prostate carcinoma cell line, and immersion fixed rat mesenchymal stem cells
Sample: Immersion fixed DU145 human prostate carcinoma cell line, and immersion fixed rat mesenchymal stem cells
Knockout Validated
Integrin alphaV/CD51
is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in
Integrin alphaV/CD51 knockout A549 cell line.
Simple Western
20 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line
Sample: HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha V/CD51
Alternate Names
CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA
Gene Symbol
ITGAV
UniProt
Additional Integrin alpha V/CD51 Products
Product Documents for Human/Mouse/Rat Integrin alphaV/CD51 Antibody
Product Specific Notices for Human/Mouse/Rat Integrin alphaV/CD51 Antibody
For research use only
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