| Specificity: |
Detects a synthetic peptide specific for human OPA1 around amino acid 200 in Direct ELISA.
Applications for Human OPA1 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
MCF-7 human breast cancer cell line and MDA-MB-231 human breast cancer cell line |
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Simple Western |
100 µg/mL |
MDA-MB-231 human breast cancer cell line |
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Immunohistochemistry |
3-25 µg/mL |
Immersion fixed paraffin-embedded sections of human kidney |
Scientific Data Examples for Human OPA1 Antibody
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Western Blot
Detection of Human OPA1 by Western Blot.
Western Blot shows lysates of MCF‑7 human breast cancer cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for OPA1 at approximately 80 and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunohistochemistry
Detection of OPA1 in Human Kidney.
OPA1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the mitochondrial membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Simple Western
Detection of Human OPA1 by Simple WesternTM.
Simple Western shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for OPA1 at approximately 90 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from cell culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human OPA1 Antibody
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