| Specificity: |
Detects a synthetic peptide corresponding to residues near amino acid 500 of human SLC1A5 protein by Direct ELISA.
Applications for Human SLC1A5 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
HCT-116 human colorectal carcinoma cell line |
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Immunohistochemistry |
0.1-10 µg/mL |
Immersion fixed paraffin-embedded sections of human prostate |
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Immunocytochemistry |
3-25 µg/mL |
Immersion fixed RT-4 human bladder carcinoma cell line and SW480 human colorectal adenocarcinoma cell line |
Scientific Data Examples for Human SLC1A5 Antibody
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Western Blot
Detection of Human SLC1A5 by Western Blot.
Western Blot shows lysates of HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11603) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SLC1A5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunocytochemistry
Detection of SLC1A5 in RT-4 cells and SW480 cells.
SLC1A5 was detected in immersion fixed RT-4 human bladder carcinoma cell line (Positive) and SW480 human colorectal adenocarcinoma cell line (Positive) using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunohistochemistry
Detection of SLC1A5 in Human Prostate.
SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11603) at 0.1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of glandular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human SLC1A5 Antibody
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