| Specificity: |
Detects a synthetic peptide specific for human SLC1A5 around amino acid 520 in Direct ELISA.
Applications for Human SLC1A5 Antibody
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Recommended Concentration
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Sample |
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Immunohistochemistry |
1-10 µg/mL |
Immersion fixed paraffin-embedded sections of human prostate |
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Immunocytochemistry |
1-10 µg/mL |
Immersion fixed SW480 human colorectal adenocarcinoma cell line |
Scientific Data Examples for Human SLC1A5 Antibody
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Immunohistochemistry
Detection of SLC1A5 in Human Prostate.
SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11628) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Immunocytochemistry
Detection of SLC1A5 in SW480 Human Cell Line.
SLC1A5 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11628) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human SLC1A5 Antibody
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