Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 287403
Product Specifications
Immunogen
E. coli-derived recombinant human SOX3
Met1-Leu230
Accession # P41225
Met1-Leu230
Accession # P41225
Specificity
Detects human SOX3 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human SOX2, 7, 10, or 17 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human SOX3 Antibody
SOX3 in NTera‑2 Human Cell Line.
SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Mouse Anti-Human SOX3 Monoclonal Antibody (Catalog # MAB2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green, upper panel; Catalog # NL009) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SOX3 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB2569 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX3
Long Name
Transcription Factor SOX3
Alternate Names
SOXB
Entrez Gene IDs
6658 (Human)
Gene Symbol
SOX3
UniProt
Additional SOX3 Products
Product Documents for Human SOX3 Antibody
Product Specific Notices for Human SOX3 Antibody
For research use only
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