Species Reactivity:

Human

Immunogen:

E. coli-derived recombinant human T-bet
Glu326-Asp535
Accession # Q9UL17

Applications:

Simple Western, Western Blot

Label:

Unconjugated

Source:

Monoclonal Mouse IgG_2B Clone # 1091005

Specificity:

Detects recombinant human T-bet protein in Direct ELISA.

View Full Technical Details for Human T-bet/TBX21 Antibody

Technical Data
Citations / Reviews

Applications for Human T-bet/TBX21 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	Jurkat human acute T cell leukemia cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line
Simple Western	20 µg/mL	Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human T-bet/TBX21 Antibody

	Western Blot Detection of Human T‑bet/TBX21 by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, SUP‑T1 human T cell lymphoblastic lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for T‑bet/TBX21 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Simple Western Detection of Human T‑bet/TBX21 by Simple Western^TM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for T‑bet/TBX21 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11637). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Item	Value
Long Name	T-box transcription factor TBX21
Entrez Gene IDs	30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names	T-PET, T-bet, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, TBETT-PET, TBLYM, TBLYMT-cell-specific T-box transcription factor T-bet, TBX21, Tbet, Transcription factor TBLYM

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Human T-bet/TBX21 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11637

Key Product Details

Applications for Human T-bet/TBX21 Antibody

Scientific Data Examples for Human T-bet/TBX21 Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: T-bet/TBX21

Customer Reviews for Human T-bet/TBX21 Antibody

Product Documents for Human T-bet/TBX21 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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