| Specificity: |
Detects a synthetic peptide specific for human TOMM20 containing amino acid 55 in Direct ELISA.
Applications for Human TOMM20 Antibody
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Recommended Concentration
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Sample |
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Western Blot |
2 µg/mL |
HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line |
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Immunohistochemistry |
3-25 µg/mL |
Immersion fixed paraffin-embedded sections of human lung and testis |
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Immunocytochemistry |
3-25 µg/mL |
Immersion fixed A549 human lung carcinoma cell line |
Scientific Data Examples for Human TOMM20 Antibody
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Immunocytochemistry
Detection of TOMM20 in A549 Human Cell Line.
TOMM20 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the mitochondrion membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot
Detection of Human TOMM20 by Western Blot.
Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TOMM20 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Immunohistochemistry
Detection of TOMM20 in Human Lung.
TOMM20 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of bronchial epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Immunohistochemistry
Detection of TOMM20 in Human Testis.
TOMM20 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial and Leydig cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from hybridoma culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human TOMM20 Antibody
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