Human Ubiquitin Antibody

Bio-Techne includes R&D Systems | Catalog # A-106

Discontinued Product
A-106 has been discontinued and is replaced by A-104.

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # FK2

View all Ubiquitin Antibodies »

Product Summary for Human Ubiquitin Antibody

Immunogen

Human Ubiquitin

Specificity

This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and polyubiquitinated proteins in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Ubiquitin Antibody

20 µg of cell lysates produced from HCT-116 cells ± 5 uM epoxomicin treatment (4 hours) were resolved on a 4-20% SDS-PAGE gel. Western blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Monoubiquitin and polyubiquitinated proteins were easily visualized with a 10 second exposure using E.C.L. reagents.

Applications for Human Ubiquitin Antibody

Application
Recommended Usage

Immunoprecipitation

Western Blot

0.1-0.5 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Boston Bio will fill in the Formulation info

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast. In mammals, four ubiquitin genes encode for two ubiquitin­ribosomal fusion proteins and two polyubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C­terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi­step process that requires three enzymes: a ubiquitin­activating (E1) enzyme, a ubiquitin­conjugating (E2) enzyme, and a ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP­dependent Proteolysis Factor 1 (APF­1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post­endocytic sorting.

References

Haas A.L. & Bright P.M. (1985) J. Biol. Chem 260: 12464 Guarino L.A. et al. (1995) Cell 80: 301 Takada K. et al. (1995) Eur. J. Biochem. 233: 42

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

Additional Ubiquitin Products

Product Documents for Human Ubiquitin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Ubiquitin Antibody

Not for use in humans

For research use only

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