Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71038
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Western-Blot-NBP2-71038-img0001.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]")
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Western-Blot-NBP2-71038-img0003.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2B5
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human IDO1(NP_002155) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
45.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO.![Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71038-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]")
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]
Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.![Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71038-img0005.jpg "Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]")
Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]
Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038] - Staining of paraffin-embedded Human lymphoma tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Western-Blot-NBP2-71038-img0002.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)Azide and BSA Free [NBP2-71038]")
![Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]](https://resources.bio-techne.com/images/products/Indoleamine-2-3-dioxygenase-IDO-Antibody-OTI2B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71038-img0006.jpg "Immunohistochemistry: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) - Azide and BSA Free [NBP2-71038]")
Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Indoleamine 2,3-dioxygenase/IDO
Alternate Names
3dioxygenase, IDO, IDO1, INDO, Indoleamine 2
Gene Symbol
IDO1
Additional Indoleamine 2,3-dioxygenase/IDO Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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