IRE1 [p Ser724] Antibody (PSH03-35)

Western Blot: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774]

Western Blot: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774] - Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (NBP3-32774) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 5 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32774) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774]

Immunohistochemistry: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-IRE1 antibody (NBP3-32774) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32774) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774]

Immunocytochemistry/ Immunofluorescence: IRE1 [p Ser724] Antibody (PSH03-35) [NBP3-32774] - Immunocytochemistry analysis of Jurkat cells treated with 100nM Calyculin A for 30 minutes labeling IRE1 with Rabbit anti-IRE1 antibody (NBP3-32774) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRE1 antibody (NBP3-32774) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.