LASS6 Antibody - BSA Free

Knockdown Validated: LASS6 Antibody - BSA Free [NBP1-76965]

Knockdown Validated: LASS6 Antibody [NBP1-76965] - CerS6 (LASS6)-dependent C16-ceramide generation mediates stress response to serum starvation. Serum withdrawal elevates CerS6 in cultured cells with concomitant increase of p53 and p21 while CerS6 siRNA silencing prevents the p53 and p21 accumulation in response to serum withdrawal. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06650-y) licensed under a CC-BY license.

Western Blot: LASS6 Antibody - BSA Free [NBP1-76965] -

Western Blot: LASS6 Antibody - BSA Free [NBP1-76965] - CerS6-dependent C16-ceramide generation mediates stress response to serum starvation. a Serum withdrawal elevates CerS6 in cultured cells with concomitant increase of p53 & p21 while CerS6 siRNA silencing prevents the p53 & p21 accumulation in response to serum withdrawal. b CerS6 siRNA silencing partially rescues G0/G1 cell cycle arrest upon serum starvation. c Serum withdrawal elevates C16-ceramide in A549 cells (48 h post-withdrawal); this effect was abrogated upon CerS6 silencing (mean ± SE; n = 3; *P < 0.001; #P < 0.05, ANOVA). d p53 is pulled down in complex with C16-ceramide from serum-starved A549 cells but not from serum-starved cells with silenced (siRNA) CerS6 (mean ± SE; n = 3; *P < 0.00001, ANOVA). e Proposed mechanism of p53 regulation by C16-ceramide: cellular stress elevates CerS6 in endoplasmic reticulum, leading to generation of C16-ceramide (C16); ceramide binds to p53, preventing its interaction with MDM2; this halts the p53 ubiquitination & degradation; resulting accumulation of p53 leads to its nuclear translocation & transcriptional activation of stress response proteins Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30297838), licensed under a CC-BY license. Not internally tested by Novus Biologicals.