LIN-28B Antibody (7D7F7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16815

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16815-100ul
NBP3-16815-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7D7F7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIN-28B Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human LIN-28B (Q6ZN17). MAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVAN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN-28B Antibody (7D7F7)

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]

Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]

Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for LIN-28B Antibody (7D7F7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIN-28B

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Gene Symbol

LIN28B

Additional LIN-28B Products

Product Documents for LIN-28B Antibody (7D7F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIN-28B Antibody (7D7F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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