Ly-6A/E Antibody (SR3456)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27773

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-27773-100ul
NBP3-27773-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3456

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ly-6A/E Antibodies »

Product Summary for Ly-6A/E Antibody (SR3456)

Immunogen

A synthesized peptide derived from human Ly-6A/E (Uniprot #: P05533)

Specificity

Detects endogenous levels of total Ly-6A/E

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ly-6A/E Antibody (SR3456)

Western Blot: Ly-6A/E Antibody (SR3456) [NBP3-27773] -

Western Blot: Ly-6A/E Antibody (SR3456) [NBP3-27773] - Western blot analysis of mouse kidney lysate.

Applications for Ly-6A/E Antibody (SR3456)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6A/E

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Alternate Names

Ly 6A/E, Ly6A.2, Ly6A/E, Ly6E.1, Lymphocyte antigen 6 complex locus A, Lymphocyte antigen 6 complex locus E, Retinoic acid induced gene E, RIGE, SCA1, SCA-1, SCA2, Stem cell antigen 1, Stem cell antigen 2, T cell activating protein, TAP, Thymic shared antigen 1, TSA1

Gene Symbol

LY6A

Additional Ly-6A/E Products

Product Documents for Ly-6A/E Antibody (SR3456)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ly-6A/E Antibody (SR3456)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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