MCM2 [p Ser139] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16941

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM2 Antibodies »

Product Summary for MCM2 [p Ser139] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around S139 of human MCM2 (NP_004517.2). YDSDE

Modification

p Ser139

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM2 [p Ser139] Antibody - Azide and BSA Free

Western Blot: MCM2 [p Ser139] AntibodyAzide and BSA Free [NBP3-16941]

Western Blot: MCM2 [p Ser139] AntibodyAzide and BSA Free [NBP3-16941]

Western Blot: MCM2 [p Ser139] Antibody [NBP3-16941] - Western blot analysis of extracts of HeLa cells, using MCM2 antibody (NBP3-16941) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for MCM2 [p Ser139] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM2

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 [p Ser139] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM2 [p Ser139] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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