MDM2/HDM2 [p Ser166] Antibody (JE64-20)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32777

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32777
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE64-20

Concentration

1 mg/ml

View all MDM2/HDM2 Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding S166 of human MDM2/HDM2. (Uniprot: Q00987)

Modification

p Ser 166

Localization

Nucleus, nucleoplasm, nucleolus, cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDM2/HDM2 [p Ser166] Antibody (JE64-20)

MDM2/HDM2 [p Ser166] Antibody (JE64-20)

Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777]

Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: A549 treated with pp for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 55 kDa
Observed band size: 110/140 kDa

Exposure time: 57 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for MDM2/HDM2 [p Ser166] Antibody (JE64-20)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDM2/HDM2

MDM2 is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Gene Symbol

MDM2

Additional MDM2/HDM2 Products

Product Documents for MDM2/HDM2 [p Ser166] Antibody (JE64-20)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MDM2/HDM2 [p Ser166] Antibody (JE64-20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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