N-WASP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23279

Novus Biologicals, part of Bio-Techne
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NBP3-23279
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Summary for N-WASP Antibody

Immunogen

N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP. [UniProt# O00401]

Specificity

This antibody detects a 65 kDa protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa WASP protein in Jurkat cell lysate.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for N-WASP Antibody

N-WASP Antibody

Western Blot: N-WASP Antibody [NBP3-23279] -

Western Blot: N-WASP Antibody [NBP3-23279] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

Applications for N-WASP Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for N-WASP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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