N-WASP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23285
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for N-WASP Antibody
Immunogen
Unphosphorylated N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484. [UniProt# O00401]
Specificity
This antibody was cross-adsorbed to phospho-N-WASP (S484/S485) peptide (without carrier) then affinity purified using unphosphorylated N-WASP (S484/S485) peptide (without carrier). The antibody detects a 65 kDa protein on SDS-PAGE immunoblots of rat brain and SKN-SH cell lysates that are treated with alkaline phosphatase. Only low levels of unphosphorylated WASP or N-WASP are detected in untreated rat brain, Jurkat, and A431 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for N-WASP Antibody
Western Blot: N-WASP Antibody [NBP3-23285] -
Western Blot: N-WASP Antibody [NBP3-23285] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).
Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23285] -
Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23285] - Immunocytochemical labeling of phospho- and unphospho-N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of blocking peptide.Applications for N-WASP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: N-WASP
Long Name
Neural Wiskott-Aldrich Syndrome Protein
Alternate Names
NWASP, TRS4, WASL
Gene Symbol
WASL
Additional N-WASP Products
Product Documents for N-WASP Antibody
Product Specific Notices for N-WASP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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