NCAM-1/CD56 Antibody (JF1021)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66968

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Multiplex Immunoassay, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF1021

Concentration

1 mg/ml

View all NCAM-1/CD56 Antibodies »

Product Summary for NCAM-1/CD56 Antibody (JF1021)

Immunogen

Synthetic peptide within C-terminal human NCAM-1/CD56. (SwissProt: P13591 Human; SwissProt: P13595 Mouse)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCAM-1/CD56 Antibody (JF1021)

Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Immunocytochemistry/ Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for NCAM-1/CD56 Antibody (JF1021)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Multiplex Immunoassay

Multiplex IHC: 1:1000

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (JF1021)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCAM-1/CD56 Antibody (JF1021)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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