NDP52 Antibody (PSH02-33)

Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636]

Western Blot: NDP52 Antibody (PSH02-33) [NBP3-32636] - Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (NBP3-32636) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Raji cell lysate
Lane 4: THP-1 cell lysate
Lane 5: Huh7 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Saos-2 cell lysate
Lane 8: RAW264.7 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 3 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32636) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: NDP52 Antibody (PSH02-33) [NBP3-32636]

Flow Cytometry: NDP52 Antibody (PSH02-33) [NBP3-32636] - Flow cytometric analysis of HeLa cells labeling NDP52.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32636, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).