NDP52 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19499
![Western Blot: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Western-Blot-NBP2-19499-img0002.jpg "Western Blot: NDP52 Antibody [NBP2-19499]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Chimpanzee (97%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region; Golgi apparatus; cytoskeleton
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NDP52 Antibody
![Western Blot: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Western-Blot-NBP2-19499-img0007.jpg "Western Blot: NDP52 Antibody [NBP2-19499]")
Western Blot: NDP52 Antibody [NBP2-19499]
Western Blot: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein by western blot analysis.Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody.
The ACTB was used as internal control, shown at the bottom panel. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
![Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19499-img0010.jpg "Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP2-19499]")
Immunocytochemistry/ Immunofluorescence: NDP52 Antibody [NBP2-19499]
Immunocytochemistry/Immunofluorescence: NDP52 Antibody [NBP2-19499] - NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI.Scale bar= 10 um.![Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Immunohistochemistry-Paraffin-NBP2-19499-img0003.jpg "Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499]")
Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499]
Immunohistochemistry-Paraffin: NDP52 Antibody [NBP2-19499] - H441 xenograft, using NDP52 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Western-Blot-NBP2-19499-img0006.jpg "Western Blot: NDP52 Antibody [NBP2-19499]")
![Immunoprecipitation: NDP52 Antibody [NBP2-19499]](https://resources.bio-techne.com/images/products/NDP52-Antibody-Immunoprecipitation-NBP2-19499-img0009.jpg "Immunoprecipitation: NDP52 Antibody [NBP2-19499]")
Applications for NDP52 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NDP52
Alternate Names
Antigen nuclear dot 52 kDa protein, calcium binding and coiled-coil domain 2, calcium-binding and coiled-coil domain-containing protein 2, NDP52MGC17318, Nuclear domain 10 protein 52, Nuclear domain 10 protein NDP52, Nuclear dot protein 52
Gene Symbol
CALCOCO2
UniProt
Additional NDP52 Products
Product Documents for NDP52 Antibody
Product Specific Notices for NDP52 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.