NuMA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-174

Novus Biologicals, part of Bio-Techne
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NB500-174
NB500-174SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mammal, Marsupial, Primate

Cited:

Human, Mouse, Rat, Mammal, Marsupial

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NuMA Antibodies »

Product Specifications

Immunogen

Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.

Reactivity Notes

Use in Marsupial reported in scientific literature (PMID:29185983).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NuMA Antibody

Western Blot: NuMA Antibody [NB500-174]

Western Blot: NuMA Antibody [NB500-174]

Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174]

Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174]

Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - Human HeLa cells fixed with 3.5% formaldehyde and stained with NB500-174 at 1:1000. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosis.
Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human placenta using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Strong nuclear with moderate cytoplasmic immuno-reactivity of NuMA was observed in syncytiotrophoblast, cytiotrophoblasts, Hofbauer cells and the endothelial cells of chorionic villi capillaries. 10X Magnification.
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Applications for NuMA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Frozen

reported in scientific literature

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Simple Western

1:500

Western Blot

1:1000
Application Notes
In Western blot a band can be seen at 220 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NuMA

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Long Name

Nuclear Mitotic Apparatus Protein 1

Alternate Names

NMP-22, NUMA1, SP-H Antigen

Entrez Gene IDs

4926 (Human)

Gene Symbol

NUMA1

UniProt

Q14980 (Human)

Additional NuMA Products

Product Documents for NuMA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NuMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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