Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PARP10 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of Poly (ADP-ribose) Polymerase Family, member 10 using the numbering given in entry NP_116178.1 (GeneID 84875)

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARP10 Antibody

Western Blot: PARP10 Antibody [NB100-2157]

Western Blot: PARP10 Antibody [NB100-2157]

Western Blot: PARP10 Antibody [NB100-2157] - Detection of Human PARP10 on HeLa whole cell lysate using NB100-2157. PARP10 was also immunoprecipitated using other rabbit anti-PARP10 antibodies.
Immunohistochemistry-Paraffin: PARP10 Antibody [NB100-2157]

Immunohistochemistry-Paraffin: PARP10 Antibody [NB100-2157]

Immunohistochemistry-Paraffin: PARP10 Antibody [NB100-2157] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PARP10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for PARP10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 -1:2000

Immunohistochemistry-Paraffin

1:500 -1:2000

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:500-1:2500
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PARP10

PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family involved in a variety of processes which includes DNA metabolism, the DNA damage response, transcription and the maintenance of genomic stability. Although each member of the PARP family exhibits poly(ADP-ribose) polymerase activity which catalyzes the attachment of ADP-ribose units from NAD+ to substrate proteins, the endogenous substrates of many of the family members, including PARP10, remain to be identified. Recent studies have shown that PARP10 is localized to the nucleoli and associates with RNA polymerase II. PARP10 is a substrate of and is activated by CDK2 and may serve an important function in cell proliferation. Alternate names for PARP10 include poly(ADP-ribose) polymerase 10, PARP-10, and FLJ14464.

Long Name

Protein mono-ADP-ribosyltransferase PARP10

Alternate Names

ARTD10, PARP-10

Entrez Gene IDs

84875 (Human)

Gene Symbol

PARP10

UniProt

NP_116178.1 (Human)

Additional PARP10 Products

Product Documents for PARP10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PARP10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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