Pax6 Antibody (CL5414)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61411

Novus Biologicals, part of Bio-Techne
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NBP2-61411
NBP2-61411-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5414

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYW

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Pax6 Antibody (CL5414)

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411]

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411]

Immunocytochemistry/Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of HEK 293 cells using the Anti-PAX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411]

Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411]

Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of rat eye retina shows strong nuclear positivity in the inner nuclear layer and ganglion cell layer.
Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411]

Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411]

Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of mouse embryo E11 shows strong nuclear positivity in the developing brain and sensory organs.
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Applications for Pax6 Antibody (CL5414)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

Additional Pax6 Products

Product Documents for Pax6 Antibody (CL5414)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pax6 Antibody (CL5414)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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